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A simple and sensitive colorimetric aptasensor for rapid and facile detection of adenosine triphosphate (ATP) has been demonstrated here based on aptamer-based hairpin probes and unmodified gold nanoparticles (AuNPs). The hairpin probe is constructed by adding another five nucleotides to the 5′-end of an anti-ATP aptamer which can hybridize to nucleotides at the 3′-end of the aptamer, forming a hairpin-shaped...
A simple Schiff base (BMSA) prepared from salicylaldehyde and 2-(1H-benzo[d]imidazol-2-yl)aniline was evaluated as an efficient fluorescent chemosensor for the selective recognition of Al3+and Cu2+ over other common metal ions. This sensor could detect Al3+ in CH3OH/PBS with distinct emission red-shift (the detection limit 0.31μM)and Cu2+in CH3OH/Tris-HCL (the detection limit 0.54μM) with obvious...
Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has become an important technology for glycan analysis due to its ease of operation, short analysis time and impurity tolerance. However, the low ionization efficiency of N-glycans led to the difficulty in analyzing glycans of low abundance in complex biological samples due to the lack of basic site for protonation. Therefore,...
Fluorescence anisotropy, known as a simple, homogeneous and cost-effective analytical technology, is an invaluable technique for studying the micro-environmental changes of the dye associated with the molecular interactions. An in-depth understanding of the variables affecting the fluorescence anisotropy signal can facilitate better experimental designs to effectively improve the analytical performance...
Random compartmentalization of cells by common droplet formation methods, i.e., T-junction and flow-focusing, results in low occupancy of droplets by single cells. To resolve this issue, a fluorescence-activated droplet formation method was developed for the on-command generation of droplets and encapsulation of single cells. In this method, droplets containing one cell were generated by switching...
Rapid mixing of highly viscous solutions is a great challenge, which helps to analyze the reaction kinetics in viscous liquid phase, particularly to discover the folding kinetics of macromolecules under molecular crowding conditions mimicking the conditions inside cells. Here, we demonstrated a novel microfluidic mixer based on Dean flows with three-dimensional (3D) microchannel configuration for...
A novel method based on TurborFlow online solid phase extraction (SPE) combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been established for simultaneous screening and confirmation of 88 wide-range veterinary drugs belonging to eight families (20 sulfonamides, 7 macrolides, 15 quinolones, 8 penicillins, 13 benzimidazoles, 4 tetracyclines, 2 sedatives, and 19 hormones) in...
In this study, magnetic solid phase extraction coupled with in-situ derivatization (MSPE-ISD) was established for the determination of hexanal and heptanal in human urine. 2,4-Dinitrophenylhydrazine (DNPH) was used as the derivatization reagent that was adsorbed onto the surface of magnetite/silica/poly(methacrylic acid-co-ethylene glycol dimethacrylate) (Fe 3 O 4 /SiO 2 /P(MAA-co-EGDMA))...
The electron-transfer based quenching effect of commonly encountered transition metal ions on the photoluminescence of grapheme quantum dots (GQDs) was for the first time investigated, and was found to be associated with electron configuration of the individual metal ion. Ethylene diamine tetraacetic acid (EDTA), the metal ion chelator, can competitively interact with metal ions to recover the quenched...
We reported a simple and reusable fluorescent sensor for heme proteins based on the electrospun nanofibrous membrane doped with a fluorescent conjugated polymer P. The sensor showed favorable fluorescence sensing performance towards the heme proteins, including hemoglobin (Hb), myoglobin (Mb) and cytochrome c (Cyt c). The surface wettability and sensing performance of the electrospun nanofibrous membrane...
Analysis of fast biochemical reactions requires rapid mixing of solutions. Micromixers can achieve uniform mixing of solutions in a short time and have been recognized as an attractive tool to analyze fast reactions. However, it is still a challenge to design mixers with simple structure and short dead time. Here, a zigzag turbulent micromixer was developed with a rapid mixing time of 16μs at sample...
A novel affinity purification method for lysozyme (LZM) based on functionalized magnetic microspheres was developed. Tris(hydroxymethyl)aminomethane (Tris)-modified magnetic microspheres with specific affinity toward LZM were prepared using Tris as ligand and silica-coated magnetic microshperes as support. Transmission electron microscopy and magnetic property measurement results showed that the Tris-modified...
The purpose of this study was to establish a simple and sensitive analytical method for lysozyme using Plasmon Resonance Light-Scattering (PRLS) technique with Gold Nanoparticles (AuNPs) as the probe. Nanomolar level of lysozyme induced AuNPs aggregation with enhanced PRLS. For 1.4nM citrate-capped AuNPs (13nm in diameter), the linear range of the calibration curve was 15-50nM with a detection limit...
Symphytum officinale L. (comfrey) is a medicinal plant commonly used in decoctions and aliments. Besides therapeutic bioactive compounds present in the herb, it is found to contain hepatotoxic pyrrolizidine alkaloids (PAs), such as lycopsamine and others. In the present study, PAs such as lycopsamine, echimidine and lasiocarpine were determined using electrospray liquid chromatography–mass spectrometry...
Tussilago farfara (Kuan Donghua) is an important Chinese herbal medicine which has been shown to contain many bioactive compounds and widely used to relieve cough and resolve phlegm. However, besides therapeutic bioactive compounds, this herb has been found to contain toxic pyrrolizidine alkaloids (PAs), mainly senkirkine and traces of senecionine. In this report, conditions for microwave-assisted...
Enzyme activity and partition behavior in aqueous biphasic systems (ABSs) consisting of ionic liquid (IL) and salt (IL-ABSs) were investigated to increase our understanding of IL-ABSs and shed light on their application potential as enzyme extraction system. With horseradish peroxidase (HRP) as the model enzyme, several effects of alkylimidazolium chloride–K 2 HPO 4 ABSs on activity...
Simple and sensitive methods for the determination of glycogen are desirable. This study was to establish the determination of glycogen based on the enhancement of the light-scattering intensity of gold nanoparticles (AuNPs) at nanogram per milliliter levels. Glycogen enhancement was independent of temperature and was highest at pH 7. The enhancement was linear from 10 to 800ngmL −1 with a...
1-Butyl-3-methylimidazolium tetrafluoroborate ([Bmim]BF 4 ), an ionic liquid (IL) immiscible with water, was used as a new type of solvent and porogen for the preparation of molecularly imprinted silica. The new imprinted silica was prepared by a sacrificial spacer molecular imprinting approach with testosterone as template molecule. The new covalent monomer-template complex used in the imprinting...
An aqueous two-phase system (ATPS) of poly(ethylene glycol) (PEG)/K 2 HPO 4 coupled with high performance liquid chromatography (HPLC) method was developed for the separation and determination of morphine in compound liquorice. Morphine and its analogs were used as model compounds to investigate influence of various factors on extraction behaviors of ATPS, such as the types and concentrations...
Multiphoton-excited fluorescence by diode laser of continuous wave was uniquely developed for capillary electrophoresis to determine aniline species metabolized from pesticides. To achieve 2-photon excitation fluorescence, derivatization procedure was performed using fluorescein isothiocyanate (FITC). The concentration ratio of FITC to the analytes was discussed for quantitative analysis. Several...
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