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A fully automated stepwise injection spectrophotometric method for determination of antipyrine in saliva (agent for non-invasive assessment of the activity of the drug metabolizing system in hepatocytes) has been developed. The method is based on the antipyrine derivatization by nitrite-ion dispersive liquid–liquid microextraction (DLLME) of formed 4-nitrosoantipyrine with subsequent UV–vis spectrophotometric...
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