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A new simple and extremely versatile zymography method based on transesterification and esterification reactions was developed in this work. The method consists in building a transesterification or esterification reaction medium according to the goal of the research. Since commercial enzymes and crude extracts commonly consist in pools of proteins, this methodology provides a means to determine which...
In recent years, the production of renewable fuels such as biodiesel has attracted considerable interest as an alternative to fossil fuels. The biodiesel production via enzymatic processes has considerable advantages over conventional alkali-catalyzed processes. Fungal lipases, which include lipases from filamentous fungi and yeasts, are among the most used biocatalysts in enzymatic synthesis of biodiesel...
Ethanol is one of the main biobased molecule produced worldwide, mainly from corn and other starchy crops. In the past few years, one promising technology that has been claimed to reduce capital and operational costs of industrial plants and increase overall yields for ethanol is named ‘cold starch hydrolysis’. The saccharification is carried out at low temperatures (under starch gelatinization point)...
The aim of this study was to investigate a new process of enzyme/enzyme hydroesterification for biodiesel production using a low-cost acid oil (10.5wt.% acidity) from macauba (Acrocomia aculeata) pulp as raw material. The ethyl esters were produced by the hydrolysis of the macauba oil using vegetable enzyme (VE) obtained from dormant castor seeds followed by esterification of the released free fatty...
The conversion of renewable feedstocks into a range of commercial products and energy is the principle underlying the concept of biorefinery. In this work, two agroindustrial by-products from babassu were used as unconventional biomasses to develop a sustainable process. Babassu cake was used to produce a multienzyme complex, which was able to hydrolyse starch granules and to release free amino nitrogen...
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