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This paper describes a multiplexed enzyme-based assay within a microfluidic device using shape-coded poly(ethylene glycol) (PEG) hydrogel microparticles. A microfluidic device was constructed by serially connecting two patterning chambers and a microfilter-integrated detection chamber through a Y-shaped microchannel. Various shapes and sizes of hydrogel microparticles entrapping different enzymes...
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