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The GAL1 gene encoding galactokinase was disrupted in a recombinant Saccharomyces cerevisiae strain in which production of LK8 protein, a kringle fragment of human apolipoprotein, is under the control of GAL1 promoter. Null mutation of the HXK2 gene was introduced further in the gal1Δ strain to relieve glucose repression. A pattern for LK8 expression was compared for the two recombinant S. cerevisiae...
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