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In this study, an aptamer-based fluorescent sensing platform using triple-helix molecular switch (THMS) was developed for the pesticide screening represented by acetamiprid. The THMS was composed of two tailored DNA probes: a label-free central target specific aptamer sequence flanked by two arm segments acting as a recognition probe; a hairpin-shaped structure oligonucleotide serving as a signal...
We designed a turn-on fluorescence assay for glyphosate based on the fluorescence resonance energy transfer (FRET) between negatively charged CdTe quantum dots capped with thioglycolic acid (TGA-CdTe-QDs) and positively charged gold nanoparticles stabilized with cysteamine (CS-AuNPs). Oppositely charged TGA-CdTe-QDs and CS-AuNPs can form FRET donor–acceptor assemblies due to electrostatic interactions,...
With citrate-coated Au nanoparticles as colorimetric probe, a novel visual method for rapid assay of organophosphorus pesticides has been developed. The assay principle is based on catalytic hydrolysis of acetylthiocholine into thiocholine by acetylcholinesterase, which induces the aggregation of Au nanoparticles and the color change from claret-red to purple or even grey. The original plasmon absorption...
Water-soluble CdTe quantum-dots (QDs) of different sizes capped with thioglycolic acid (TGA) were synthesized via a microwave-assisted method. It was found that CdTe QDs, as a kind of sensitizer, could enhance the chemiluminescence (CL) emission from the redox reaction of SO 32− with Ce(IV) in acidic medium. In combination with the flow injection technique, the effects of reactant concentrations,...
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