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Rutin-degrading enzymes (RDEs) specifically hydrolyze the glycosidic linkages of rutin, producing quercetin and rutinose. Here we report a reliable and sensitive polyacrylamide gel electrophoresis and staining method for the detection of RDE isozymes, which is based on the aqueous solubility difference between rutin and quercetin, as well as the ultraviolet absorbance of quercetin. With this novel...
Fifteen sugar osazones were synthesized and evaluated for their use as matrix-assisted laser desorption/ionization matrices. Various carbohydrate samples were analyzed, indicating thatd- orl-arabinosazone, prepared fromd- orl-arabinose and phenylhydrazine, gave the overall best performance. The arabinosazones facilitated superior analyses in providing a highly uniform distribution of sample molecules...
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