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Bradyrhizobium sp. (Lupinus) strains 750 and IM-43B, possessing nitrate reductase activity (NRA) were used to inoculate Lupinus albus cv. Multolupa plants. Bacteroids from root nodules were sonicated and sedimented to separate membranes from cytosolic fractions. Our results confirmed the existence of constitutive NRA mostly in the membranes, but soluble and membrane-bound NR forms were also induced...
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