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A defined medium and fed-batch feeding process for the production of a yeast biocatalyst, developed at the 23-L scale, was scaled up to the 600-L pilot scale. Presterilized 100-L-vol plastic bags were implemented for the pilotscale nutrient feeding. Medium of increased concentration Ôqs implemented at the pilot scale, and equivalent dry cell weights were reached with a medium 80% more concentrated...
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