Search results for: Jonas Perales

Items from 1 to 20 out of 67 results

article

Defensin‐like peptides from Capsicum chinense induce increased ROS, loss of mitochondrial functionality, and reduced growth of the fungus Colletotrichum scovillei

Larissa Maximano Resende, Érica Oliveira Mello, Ana Eliza Zeraik, Arielle Pinheiro Bessiati Fava Oliveira, more

Pest Management Science > 80 > 7 > 3567 - 3577

In the present study, we identified and characterized two defensin‐like peptides in an antifungal fraction obtained from Capsicum chinense pepper fruits and inhibited the growth of Colletotrichum scovillei, which causes anthracnose. AMPs were extracted from the pericarp of C. chinense peppers and subjected to ion exchange, molecular exclusion, and reversed‐phase in a high‐performance liquid chromatography...

article

Differential proteomic comparison of breast cancer secretome using a quantitative paired analysis workflow

Giselle Villa Flor Brunoro, Paulo Costa Carvalho, Valmir C. Barbosa, Dante Pagnoncelli, more

BMC Cancer > 2019 > 19 > 1 > 1-12

Background Worldwide, breast cancer is the main cause of cancer mortality in women. Most cases originate in mammary ductal cells that produce the nipple aspirate fluid (NAF). In cancer patients, this secretome contains proteins associated with the tumor microenvironment. NAF studies are challenging because of inter-individual variability. We introduced a paired-proteomic shotgun strategy that relies...

article

Proteomic profile, biological activities and antigenic analysis of the venom from Bothriopsis bilineata smaragdina (“loro machaco”), a pitviper snake from Peru

Carolina Rego Rodrigues, André Teixeira-Ferreira, Frey Francisco Romero Vargas, Clara Guerra-Duarte, more

Journal of Proteomics > 2018 > 187 > C > 171-181

In order to determine Bothriopsis bilineata smaragdina venom (BbsV) composition, proteomic approaches were performed. Venom components were analyzed by RP-HPLC, SDS- PAGE and nano LC on line with LTQ Orbitrap XL. Results showed a total of 189 identified proteins, grouped into 11 different subgroups, which include snake venom metalloproteinases (SVMPs, 54.67%), snake C-type lectins (Snaclecs, 15.78%),...

article

Soybean seed coat chitinase as a defense protein against the stored product pest Callosobruchus maculatus

Nadia CM Silva, Jamile G Conceição, Kayan Eudorico Ventury, Leonardo FR De Sá, more

Pest Management Science > 74 > 6 > 1449 - 1456

BACKGROUND Chitinases (EC 3.2.1.14) are enzymes involved in the breaking of the β‐1,4‐glycosidic linkages of chitin. In insects, chitin is present mainly in the cuticle and in peritrophic membranes and peritrophic gel. Enzymes with the potential to damage peritrophic membranes and gel, such as chitinase, have been associated with plant defense systems. Identification and characterization of seed...

article

Ferritin from the haemolymph of adult ants: an extraction method for characterization and a ferromagnetic study

Eliane Wajnberg, Odivaldo C. Alves, Jonas Perales, Surza Lucia G. Rocha, more

European Biophysics Journal > 2018 > 47 > 6 > 641-653

Ferritin has been studied in many animals, plants and bacteria. The main functions of ferritin in mammals are iron concentration and stabilization, protection against oxidants and iron storage for later developmental or iron-dependent activities. Although insect ferritin plays a key role in iron transport, only a few studies to date have examined its properties and function. Ferritin isolation from...

article

Bothrops jararaca venom gland secretory cells in culture: Effects of noradrenaline on toxin production and secretion

Luciana Godoy Viana, Richard Hemmi Valente, Cíntia Scucuglia Heluany, Andreia Souza-Imberg, more

Toxicon > 2017 > 133 > C > 1-9

Primary culture of snake venom gland secretory cells could be a good model to study the mechanism(s) of toxin(s) production. These cells can produce and secrete venom to the medium with a hemorrhagic activity comparable to that induced by venom collected from snakes. Production of new venom is triggered by the sympathetic outflow, through the release of noradrenaline, but the importance of this neurotransmitter...

article

Dataset of proteins mapped on HepG2 cells and those differentially abundant after expression of the dengue non-structural 1 protein

Kíssila Rabelo, Monique R.O. Trugilho, Simone M. Costa, André T.S. Ferreira, more

Data in Brief > 2017 > 10 > C > 248-263

The data supplied in this article are related to the research article entitled “The effect of the dengue non-structural 1 protein expression over the HepG2 cell proteins in a proteomic approach” (K. Rabelo, M.R. Trugillo, S.M. Costa, B.A. Pereira, O.C. Moreira, A.T. Ferreira et al., 2016) [1]. The present article provides the inventory of peptides and proteins mapped in a hepatocyte cell line (HepG2)...

article

The effect of the dengue non-structural 1 protein expression over the HepG2 cell proteins in a proteomic approach

Kíssila Rabelo, Monique R.O. Trugilho, Simone M. Costa, Bernardo A.S. Pereira, more

Journal of Proteomics > 2017 > 152 > C > 339-354

Dengue is an important mosquito borne viral disease in the world. Dengue virus (DENV) encodes a polyprotein, which is cleaved in ten proteins, including the non-structural protein 1 (NS1). In this work, we analyzed the effect of NS1 expression in one hepatic cell line, HepG2, through a shotgun proteomic approach. Cells were transfected with pcENS1 plasmid, which encodes the DENV2 NS1 protein, or the...

article

Proteomic analysis of the secretome of HepG2 cells indicates differential proteolytic processing after infection with dengue virus

Marjolly B. Caruso, Monique R.O. Trugilho, Luiza M. Higa, André S. Teixeira-Ferreira, more

Journal of Proteomics > 2017 > 151 > C > 106-113

Secretome analysis can be described as a subset of proteomics studies consisting in the analysis of the molecules secreted by cells or tissues. Dengue virus (DENV) infection can lead to a broad spectrum of clinical manifestations, with the severe forms of the disease characterized by hemostasis abnormalities and liver injury. The hepatocytes are a relevant site of viral replication and a major source...

article

An in-depth snake venom proteopeptidome characterization: Benchmarking Bothrops jararaca

Carolina A. Nicolau, Paulo C. Carvalho, Inácio L.M. Junqueira-de-Azevedo, André Teixeira-Ferreira, more

Journal of Proteomics > 2017 > 151 > C > 214-231

A large-scale proteomic approach was devised to advance the understanding of venom composition. Bothrops jararaca venom was fractionated by OFFGEL followed by chromatography, generating peptidic and proteic fractions. The latter was submitted to trypsin digestion. Both fractions were separately analyzed by reversed-phase nanochromatography coupled to high resolution mass spectrometry. This strategy...

article

Screening for target toxins of the antiophidic protein DM64 through a gel-based interactomics approach

Surza L.G. Rocha, Ana G.C. Neves-Ferreira, Monique R.O. Trugilho, Yamileth Angulo, more

Journal of Proteomics > 2017 > 151 > C > 204-213

DM64 is a glycosylated protein with antivenom activity isolated from the serum of the opossum Didelphis aurita. It binds non-covalently to myotoxins I (Asp49) and II (Lys49) from Bothrops asper venom and inhibits their myotoxic effect. In this study, an affinity column with immobilized DM64 as bait was used to fish potential target toxins. All ten isolated myotoxins tested were able to effectively...

article

Interaction between bradykinin B2 and Ang-(1–7) Mas receptors regulates erythrocyte invasion by Plasmodium falciparum

Leandro de Souza Silva, Diogo de Barros Peruchetti, Claudio Teixeira Ferreira-Da Silva, André Teixeira Ferreira-DaSilva, more

Biochimica et Biophysica Acta (BBA) - General Subjects > 2016 > 1860 > 11(PA) > 2438-2444

The molecular mechanisms involved in erythrocyte invasion by malaria parasite are well understood, but the contribution of host components is not. We recently reported that Ang-(1–7) impairs the erythrocytic cycle of P. falciparum through Mas receptor-mediated reduction of protein kinase A (PKA) activity. The effects of bradykinin (BK), a peptide of the kallikrein-kinin system (KKS), can be potentiated...

article

Saccharomyces cerevisiae asparaginase II, a potential antileukemic drug: Purification and characterization of the enzyme expressed in Pichia pastoris

Luciana Facchinetti de Castro Girão, Surza Lucia Gonçalves da Rocha, Ricardo Sposina Sobral, Ana Paula Dinis Ano Bom, more

Protein Expression and Purification > 2016 > 120 > C > 118-125

Asparaginase obtained from Escherichia coli and Erwinia chrysanthemi are used to treat acute lymphocytic leukaemia and non-Hodgkin's lymphoma. However, these agents cause severe adverse effects. Saccharomyces cerevisiae asparaginase II, encoded by the ASP3 gene, could be a potential candidate for the formulation of new drugs. This work aimed to purify and characterize the periplasmic asparaginase...

article

Purification and characterization of tenerplasminin-1, a serine peptidase inhibitor with antiplasmin activity from the coral snake (Micrurus tener tener) venom

Jeilyn Vivas, Carlos Ibarra, Ana M. Salazar, Ana G.C. Neves-Ferreira, more

Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology > 2016 > 179 > C > 107-115

A plasmin inhibitor, named tenerplasminin-1 (TP1), was isolated from Micrurus tener tener (Mtt) venom. It showed a molecular mass of 6542Da, similarly to Kunitz-type serine peptidase inhibitors. The amidolytic activity of plasmin (0.5nM) on synthetic substrate S-2251 was inhibited by 91% following the incubation with TP1 (1nM). Aprotinin (2nM) used as the positive control of inhibition, reduced the...

article

Proteomic profiling of nipple aspirate fluid (NAF): Exploring the complementarity of different peptide fractionation strategies

Giselle Villa Flor Brunoro, Paulo Costa Carvalho, André Teixeira da Silva Ferreira, Jonas Perales, more

Journal of Proteomics > 2015 > 117 > Complete > 86-94

NAF is a breast fluid that is closely related to the tumor microenvironment and a valuable sample for studying breast cancer. To perform an in-depth proteomic analysis of this sample, aliquots of a single NAF digest were analyzed by the following peptide-centric fractionation strategies: a) 30-cm reversed-phase (RP) column on-line with an LTQ-Orbitrap XL; b) off-line strong cation-exchange (SCX) column;...

article

Reevaluating the Trypanosoma cruzi proteomic map: The shotgun description of bloodstream trypomastigotes

Giselle Villa Flor Brunoro, Marcelle Almeida Caminha, André Teixeira da Silva Ferreira, Felipe da Veiga Leprevost, more

Journal of Proteomics > 2015 > 115 > C > 58-65

Chagas disease is a neglected disease, caused by the protozoan Trypanosoma cruzi. This kinetoplastid presents a cycle involving different forms and hosts, being trypomastigotes the main infective form. Despite various T. cruzi proteomic studies, the assessment of bloodstream trypomastigote profile remains unexplored. The aim of this work is T. cruzi bloodstream form proteomic description. Employing...

article

Characterization of Peptides from Capsicum annuum Hybrid Seeds with Inhibitory Activity Against α-Amylase, Serine Proteinases and Fungi

Gabriela C. Vieira Bard, Viviane V. Nascimento, Suzanna F. F. Ribeiro, Rosana Rodrigues, more

The Protein Journal > 2015 > 34 > 2 > 122-129

Over the last several years, the activity of antimicrobial peptides (AMPs), isolated from plant species, against different microorganisms has been demonstrated. More recently, some of these AMPs have been described as potent inhibitors of α-amylases and serine proteinases from insects and mammals. The aim of this work was to obtain AMPs from protein extracts of a hybrid Capsicum ...

article

Cardiac protein changes in rats after soybean oil treatment: a proteomic study

Taisla Soprani, Vinicius Kuffer Uliana, Rogerio Faustino Ribeiro, Sergio Lisboa, more

Lipids in Health and Disease > 2015 > 14 > 1 > 1-9

Background Several studies show that the consumption of vegetable oils, such as soybean oil, rich in polyunsaturated fatty acids (PUFAs) has beneficial health effects by preventing or reducing the risk factors of cardiovascular diseases. While the demonstration of beneficial effects of the consumption of unsaturated fatty acids on the cardiovascular system has been proven in a macroscopic level, the...

article

Comparative proteomics of cerebrospinal fluid reveals a predictive model for differential diagnosis of pneumococcal, meningococcal, and enteroviral meningitis, and novel putative therapeutic targets

Ana Paula Cordeiro, Rosiane Aparecida Silva Pereira, Alex Chapeaurouge, Clarice Semião Coimbra, more

BMC Genomics > 2015 > 16 > 5 > 1-9

Background Meningitis is the inflammation of the meninges in response to infection or chemical agents. While aseptic meningitis, most frequently caused by enteroviruses, is usually benign with a self-limiting course, bacterial meningitis remains associated with high morbidity and mortality rates, despite advances in antimicrobial therapy and intensive care. Fast and accurate differential diagnosis...

article

Proteomic analysis of the soluble proteomes of miltefosine-sensitive and -resistant Leishmania infantum chagasi isolates obtained from Brazilian patients with different treatment outcomes

Juliana B.T. Carnielli, Hélida M. de Andrade, Simone F. Pires, Alexander D. Chapeaurouge, more

Journal of Proteomics > 2014 > 108 > C > 198-208

The mechanism of miltefosine-resistance in Leishmania spp. has been partially determined in experimental resistant lines; however, studies using clinical isolates with different miltefosine susceptibilities are still needed. In our study, we used a proteomic 2D-DIGE/MS approach to study different protein abundances in miltefosine-sensitive and -resistant Leishmania infantum chagasi isolates from visceral...

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INFONA - science communication portal

Search results for: Jonas Perales

Defensin‐like peptides from Capsicum chinense induce increased ROS, loss of mitochondrial functionality, and reduced growth of the fungus Colletotrichum scovillei

Differential proteomic comparison of breast cancer secretome using a quantitative paired analysis workflow

Proteomic profile, biological activities and antigenic analysis of the venom from Bothriopsis bilineata smaragdina (“loro machaco”), a pitviper snake from Peru

Soybean seed coat chitinase as a defense protein against the stored product pest Callosobruchus maculatus

Ferritin from the haemolymph of adult ants: an extraction method for characterization and a ferromagnetic study

Bothrops jararaca venom gland secretory cells in culture: Effects of noradrenaline on toxin production and secretion

Dataset of proteins mapped on HepG2 cells and those differentially abundant after expression of the dengue non-structural 1 protein

The effect of the dengue non-structural 1 protein expression over the HepG2 cell proteins in a proteomic approach

Proteomic analysis of the secretome of HepG2 cells indicates differential proteolytic processing after infection with dengue virus

An in-depth snake venom proteopeptidome characterization: Benchmarking Bothrops jararaca

Screening for target toxins of the antiophidic protein DM64 through a gel-based interactomics approach

Interaction between bradykinin B2 and Ang-(1–7) Mas receptors regulates erythrocyte invasion by Plasmodium falciparum

Saccharomyces cerevisiae asparaginase II, a potential antileukemic drug: Purification and characterization of the enzyme expressed in Pichia pastoris

Purification and characterization of tenerplasminin-1, a serine peptidase inhibitor with antiplasmin activity from the coral snake (Micrurus tener tener) venom

Proteomic profiling of nipple aspirate fluid (NAF): Exploring the complementarity of different peptide fractionation strategies

Reevaluating the Trypanosoma cruzi proteomic map: The shotgun description of bloodstream trypomastigotes

Characterization of Peptides from Capsicum annuum Hybrid Seeds with Inhibitory Activity Against α-Amylase, Serine Proteinases and Fungi

Cardiac protein changes in rats after soybean oil treatment: a proteomic study

Comparative proteomics of cerebrospinal fluid reveals a predictive model for differential diagnosis of pneumococcal, meningococcal, and enteroviral meningitis, and novel putative therapeutic targets

Proteomic analysis of the soluble proteomes of miltefosine-sensitive and -resistant Leishmania infantum chagasi isolates obtained from Brazilian patients with different treatment outcomes

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