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An online metal‐free weak cation exchange‐hydrophilic interaction LC/RPLC system has been developed for sensitive, high‐throughput top‐down MS. Here, we report results for analyzing PTMs of core histones, with a focus on histone H4, using this system. With just ∼24 μg on‐column of core histones (H4, H2B, H2A, and H3) purified from human fibroblasts, 41 H4 isoforms were identified, with the type and...
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