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The objective of this study was to design and develop an analytical protocol using chemical and molecular techniques to detect microorganisms able to produce biogenic amines (BAs). The protocol consists of: an initial screening by TLC (Thin Layer Chromatography) to detect BA production by microorganisms; multiplex PCR to identify decarboxylase genes in the BA-positive samples; and HPLC to quantify...
We propose a rapid method for Saccharomyces cerevisiae strain identification based on multiplex PCR analysis of polymorphic microsatellite loci. Simple DNA extraction without the use of phenol, followed by a rapid PCR procedure optimised for multiplex amplification of loci SC8132X, YOR267C and SCPTSY7 and band pattern analysis of the fragments generated by agarose and polyacrylamide gel electrophoresis,...
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