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We present the optimisation of two Y-STR multiplexes for forensic casework applications especially for the analysis of mixtures of male and female DNA. The procedure involved: (1) a new design of the PCR primers for the loci DYS389, DYS390 and DYS391 in order to improve the PCR efficiency and to reduce the length of the amplification products, and (2) the addition of PCR Enhancer to the reaction mix,...
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