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A colorimetric method for S-adenosyl-l-homocysteine hydrolase (SAHase) which uses S-adenosyl-l-homocysteine (SAH) as substrate is described. This method involves the hydrolytic conversion of SAH into adenosine (ADO) and l-homocysteine (HCY). The formation of HCY is quantified using Ellman's reagent and spectrophotometrical measured at 412 nm. Under these assay conditions, the product was followed...
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