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Alkyl-substituted Sepharose 4B prepared by the glycidyl ether method with different degrees of substitution and alkyl chain length, have been used as a non-ionic matrix for immobilization of bacterial luciferases. Exposure of hydrophobic clusters in the protein molecule was confirmed by fluorescence studies using 8-anilino-1-napthalene-sulfonate as a hydrophobic reporter. Immobilization of luciferases...
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