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Azoreductases from Bacillus sp. B29 are NADH‐dependent flavoenzymes which contain a flavin mononucleotide (FMN) as a prosthetic group and exist as homodimers composed of 23 kDa subunits. These enzymes catalyze the reductive degradation of various azo compounds by a ping‐pong mechanism. In order to determine the structure–function relationship of the azo‐dye reduction mechanism, an X‐ray crystallographic...
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