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GATA1 is considered as the “master” transcription factor in erythropoiesis. It regulates at the transcriptional level all aspects of erythroid maturation and function, as revealed by gene knockout studies in mice and by genome‐wide occupancies in erythroid cells. The GATA1 protein contains two zinc finger domains and an N‐terminal transactivation domain. GATA1 translation results in the production...
The remarkable high affinity (Kd ~ 10−15 M) of avidin/streptavidin for biotin has been extensively exploited in purification methodologies. Recently a small peptide sequence (Avi-tag) has been defined that can be specifically and efficiently biotinylated by the bacterial BirA biotin ligase. Fusion of this small peptide sequence to a protein of interest and co-expression with the BirA gene in mammalian...
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