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Objective The aim of this study was to study the effect of Bcl-2 small hairpin RNA (shRNA) enhancing methotrexate (MTX)-induced apoptosis of Raji cells. Methods Expression plasmid with Bcl-2 shRNA was transfected into Raji cells by Lipofectmine 2000 and then treated with MTX. At 48 h of transfection, the expression level of Bcl-2 mRNA and protein was evaluated by RT-PCR and immunofluorescence. MTT...
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