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A polynucleotide (or a fragment of RNA) was purified to apparent homogeneity by HPLC from mycelium of the wild strain 74A of the mould Neurospora crassa, after growth on sucrose and in the presence of saturating amounts of inorganic phosphate (Pi) for 72 hr at 30°. The M r was ca 20 000 as determined by HPLC at pH 6.8. Polynucleotide synthesis ranged from 4.0 to 6.5 μg polynucleotide per...
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