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Vascular endothelial growth factors 165 (VEGF 165 ) is the most potent and widely used pro-angiogenic factor. Here we determined optimal culture condition of recombinant human VEGF 165 (rhVEGF 165 ) in Escherichia coli (E. coli). rhVEGF 165 expression was the highest in 0.25% of l-arabinose induction concentration, at 20°C induction temperature, and for 5h induction...
This study was conducted to examine the effect of neonatal maternal separation on the hypothalamic feeding peptides expression in young female offspring. Sprague–Dawley pups were separated from dam for 3h daily during PND 1–14 (MS), or left undisturbed except routine cage cleaning (NH). Weanling female pups were housed in group and the arcuate mRNA levels of neuropeptide Y (NPY), proopiomelanocortin...
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