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An ion-pair LC–UV detection assay was developed for the determination of boanmycin (BAM) in mouse plasma. The mobile phase contained methanol, acetonitrile, 0.055 M sodium hexanesulfonate and 0.08 M acetic acid in water, with a flow rate of 0.8 mL min−1. This assay involved a rapid and simple extraction process and subsequent detection at 291 nm. The limit of detection (LOD) and the limit of quantification...
An ion-pair HPLC method with UV detection is described for the determination of boanmycin in freeze-dried pharmaceutical preparations. Chromatographic separation was achieved on a 5 μm Agilent Eclipse XDB-C18 column (150 × 4.6 mm). The mobile phase contained methanol, acetonitrile, 055 M sodium hexanesulfonate and 0.08 M acetic acid in water. UV detection was at 291 nm. The assay was validated over...
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