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A permanent growing cell line, designated as koi snout (KS), was established from snout tissues of koi utilizing the trypsin method. The KS cell line was maintained in Leibovitz's L-15 medium supplemented with 10–20% fetal bovine serum (FBS) within incubation temperatures ranging from 22 to 27°C. Maximum cell proliferation rates occurred at 27°C in Leibovitz's L-15 medium containing 10% FBS. The KS...
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