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Granulocytes from healthy subjects and from patients suffering from diabetes mellitus present differences in reactivity to stimulation with cyclic nucleotide-elevating agents. The production of reactive oxygen species (ROS) is inhibited in cells from non-diabetic subjects following such stimulation, but activated through a PKA-independent signaling pathway in granulocytes from type 1 and type 2 diabetic...
We report comparative studies on metabolic response by granulocytes from healthy subjects and Type 2 diabetic patients (T2-DM) using calorimetry and luminol-dependent chemiluminescence. Three groups of substances were used for activating or inhibiting the granulocyte reactivity: (i) opsonized zimosan particles (ZC3b), phorbol ester (PDB); (ii) cytokines (IFN-γ, GM-CSF and IL-10) and (iii) cyclic nucleotide-elevating...
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