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The M r of spinach chloroplast coupling factor 1 has been determined by sedimentation equilibrium and by light scattering to be 400 000 +/- 24 600 and 407 000 +/- 20 000, respectively. These values differ substantially from that obtained previously (325 000) and are consistent with an α 3 β 3 γδε subunit stoichiometry.
Glycolate excretion by chloroplasts is essential to the process of photorespiration. Previous transport studies on intact chloroplasts have failed to find evidence for carrier-mediated transport of glycolate. The rate of glycolate uptake, when measured by a rapid silicone oil centrifugation method, saturates at high glycolate concentrations. This rapid glycolate uptake is inhibited by pretreatment...
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