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There is wide anecdotal recognition that biological cell viability and behavior can vary significantly as a function of the source of commercial tissue culture polystyrene (TCPS) culture vessels to which those cells adhere. However, this marked material dependency is typically resolved by selecting and then consistently using the same manufacturer’s product – following protocol – rather than by investigating...
Morphology of 4,4′-dicyclohexylmethane diisocyanate–poly(tetramethylene oxide) (PTMO)–diethyltoluenediamine based poly(urethane urea) (PUU) elastomers is investigated by atomic force microscopy (AFM) and compared with elastic modulus data measured from AFM-enabled indentation, dynamic nanoindentation (nanoDMA), and dynamic mechanical analysis (DMA). These measurements highlight the effect of altering...
Pericytes surround capillary endothelial cells and exert contractile forces modulating microvascular tone and endothelial growth. We previously described pericyte contractile phenotype to be Rho GTPase- and α-smooth muscle actin (αSMA)-dependent. However, mechanisms mediating adhesion-dependent shape changes and contractile force transduction remain largely equivocal. We now report that the neutral...
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