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The signal peptide Ag85B of Bacillus Chalmette‐Guerin (BCG) was used to construct a recombinant plasmid of BCG. The BCG‐Ag85B gene and fused EBV LMP2A and BZLF1 genes were amplified and successively inserted into the Escherichia coli‐BCG shuttle‐vector pMV261. The recombinant plasmids were then amplified in E. coli DH5α and transformed into competent BCG. The expression of BZLF1 and LMP2A fusion proteins...
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