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Phenolic compounds in rosemary have shown antiproliferative/cytotoxic activity against colorectal cancer cells. The aim of this work was to study in depth the absorption and metabolism of the compounds present in a rosemary extract. An in-situ perfusion assay was performed in mice, for which samples of gastrointestinal liquid taken at different times and plasma obtained at the end of the experiment...
When using the Caco‐2 intestinal model, the low uptake, intracellular presence at low levels as well as generation of trace metabolites may limit the analysis of flavonoids. To overcome these limitations, we performed a simple but sensitive methodology based on nano‐LC‐TOF‐MS, using an on‐line trapping step. The analytical method was validated for quercetin, quercetin 3‐O‐glucoside, and quercetin...
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