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EcoP15I is a Type III restriction endonuclease requiring the interaction with two inversely oriented 5′-CAGCAG recognition sites for efficient DNA cleavage. Diverse models have been developed to explain how enzyme complexes bound to both sites move toward each other, DNA translocation, DNA looping and simple diffusion along the DNA. Conflicting data also exist about the impact of cofactor S-adenosyl-l-methionine...
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