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We report a benzo[a]phenoxazine-based far red-emitting probe which selectively senses reduced nicotinamide adenine dinucleotide (NADH) and its phosphate ester NADPH, the most indispensable coenzymes found in all living cells, with “turn-off” fluorescence response at 650 nm. The original probe can be regenerated quantitatively through oxidation using hypochlorous acid, a reactive oxygen species, which...
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