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A rapid, selective and sensitive hydrophilic interaction liquid chromatography (HILIC) coupled with tandem mass spectrometry (MS/MS) method was developed to determine 1‐13C‐l‐methionine in rat serum. Proteins in serum were precipitated using acetonitrile and the supernatant was separated after centrifugation. 1‐13C‐l‐phenylalnine was used as the internal standard. HILIC–tandem mass spectrometry analysis...
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