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Aim
In neuroendocrine cells, large dense‐core vesicles (LDCVs) undergo highly regulated pre‐fusion processes before releasing hormones via membrane fusion. Significant heterogeneity has been found for LDCV population based on the dynamics of membrane fusion. However, how the pre‐fusion status impacts the heterogeneity of LDCVs still remains unclear. Hence, we explored pre‐fusion determinants of heterogeneous...