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Patients infected human papillomavirus (HPV) is increasing all over the world, in particular the community of woman. Microarray-in-a-tube system is a novel method of integrating polymerase chain reaction (PCR) with the microarray technique and a simple and high-throughput detection system. In this work, the microarray-in-a-tube system used in the function of detection human papillomavirus genotypes...
Nanopores have been proven as versatile single-molecule sensors for individual unlabeled biopolymer detection and characterization. Besides application to next gene sequencing, proteins are becoming a prime target of investigation. In our work, single molecules of bovine serum albumin have been detected as the transient current blockages while the proteins pass through a silicon nitride nanopore....
Nanopore sensor has been identified as a versatile single molecule analytical device for its stability and sensitivity. Modification of solid-state nanopore will optimize the electrical properties when molecules transport through the nanopore after modification. Here, solid-state nanopore, about 50 nm, modified with gold nanoparticles, was fabricated in SiN membrane. λ-DNA was driven to transport...
Nanopores have become an important tool for molecule detection at single molecular level. In our work, the solid nanopore in silicon nitride membranes are fabricated and characterized by experiments. Firstly, the free-standing silicon nitride membranes are designed and etched by delicate techniques. Then, the free-standing membranes are drilled by focus ion beam. A set of nanopores with varied diameters...
In order to investigate the mechanism of the recognition of antibiotics with biomembrane, thermodynamic properties of the phosphoinositides (PIs) interacting with aminoglycoside antibiotics are studied by use of isothermal titration calorimetry. The results suggest different groups in the antibiotics have varied affinity with the PIs and neomycin B has higher affinity with phosphoinositides. PI binding...
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