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The 4-(1′-cyanoisoindolyl)phenylisothiocyanate (CIPIC) could be used as a chemiluminescence (CL) Edman-type reagent. Firstly, CL reaction conditions such as pH, buffer types, H 2 O 2 concentration and organic solvents were optimized in detail. Secondly, the CL sensitivity of the derivative of Ala was compared with fluorescence and absorbance detections. Thirdly, the structures of the...
A simple and convenient chemiluminescence (CL) method for the determination of melatonin is reported. It is based on the observation that melatonin can greatly enhance the ultra-weak CL between H2O2 and acetonitrile in alkaline solution. Light emission is intense and long-lived, and even with a simple setup, a high sensitivity could be achieved. The log–log linear range was 250nM–250μM with a 3σ detection...
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