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Most of the rice (Oryza sativa L.) storage protein glutelin genes are coordinately expressed during seed maturation, although there is considerable divergence in the 5 flanking regions between members of GluA and GluB subfamilies. Hybrid promoters among the 5 distal and proximal sequences of GluA-3 and GluB-1 genes were constructed to elucidate the regulatory mechanism(s) responsible for their...
A 45-bp proximal region of the rice glutelin promoter (-104) containing two putative cis-elements, the AACA motif and GCN4 motifs, was fused to a truncated CaMV 35S promoter (-90; -90Δ35S)/GUS. The 45-bp fragment specifically enhanced the promoter activity in endosperm tissue of transformed tobacco. A substitution mutation of the GCN4 motif reduced the promoter activity, whereas mutation of the...
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