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Based on the strong enhancement effect of nucleic acids on resonance light scattering of dequalinium chloride, the determination method for micro amounts of nucleic acids has been developed. Under the experimental conditions (5.0x10 -5 mol l -1 dequalinium, pH 7.0, at room temperature) the linear range of this assay is 0.04-10.0 μg ml -1 for calf thymus DNA and...
The large particle light scattering technique was first developed as a sensitive and convenient analysis method for microdetermination of nucleic acids by using a common spectrofluorometer. In 0.1 mol l -1 HCl, H 2 SO 4 , or HNO 3 solution, the nucleic acids can aggregate to form large particles whose dimensions are comparable to the wavelength of UV-Vis light...
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