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A new protein determination method by enhanced Rayleigh light scattering (RLS) technique has been developed. In acid condition (pH=3.60), RLS of 1,2-dihydroxyanthraquinone-3-sulfonate (Alizarin Red S) can be greatly enhanced by addition of proteins, resulting in two characteristic peaks, 360 and 505nm, respectively. The new protein assay is based on the RLS enhancement and spectrum change. The optimum...
Based on the strong enhancement effect of nucleic acids on resonance light scattering of dequalinium chloride, the determination method for micro amounts of nucleic acids has been developed. Under the experimental conditions (5.0x10 -5 mol l -1 dequalinium, pH 7.0, at room temperature) the linear range of this assay is 0.04-10.0 μg ml -1 for calf thymus DNA and...
A chlorphenamine-imprinted polymer was prepared in this study. Chromatographic analysis showed that the retentivity and selectivity of the imprinted polymer were greatly strengthened through molecular imprinting. As a consequence, chlorphenamine could be easily separated from diphenhydramine. Ionic interaction was proved to be the main power for the imprinted polymer to bind chlorphenamine. The strong...
The mixture of concanavalin A (Con A) and glycogen shows strong light scattering character. Based on it, the interaction between Con A and glycogen was studied on a common spectrofluorimeter by light scattering technique. Many factors affecting the light scattering intensity (LSI), such as pH, temperature, reaction time, ion strength and the denaturing agent of protein were studied in detail. Experimental...
The large particle light scattering technique was first developed as a sensitive and convenient analysis method for microdetermination of nucleic acids by using a common spectrofluorometer. In 0.1 mol l -1 HCl, H 2 SO 4 , or HNO 3 solution, the nucleic acids can aggregate to form large particles whose dimensions are comparable to the wavelength of UV-Vis light...
This is the first report on the determination of proteins based on the interaction with carboxyarsenazo (CAA) by Rayleigh light scattering (RLS). At pH 4, the weak RLS of CAA can be enhanced greatly by the addition of proteins, resulting in three characteristic peaks. Based on this, the interactions of CAA with nine kinds of proteins were studied and a new quantitative determination method for proteins...
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