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The mechanisms behind reduced bacterial sensitivity to drugs and the nature of this phenomenon were explored. Escherichia coli were treated with a sub-inhibitory dose or a repeatedly doubled dose of enrofloxacin (EN) in vitro. After 10 serial passages, the minimal inhibitory concentration (MIC) of EN to the bacteria was assessed by changes in expression of genes involved in the SOS response, drug...
The dynamics of a bacterial population exposed to the minimum inhibitory concentration (MIC) of an antibiotic is an important issue in pharmacological research. Therefore, a novel antibiotic susceptibility test is urgently needed that can both precisely determine the MIC and accurately select antibiotic-resistant strains from clinical bacterial populations. For this purpose, we developed a method...
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