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Acyl‐CoAs are key precursors of primary and secondary metabolism. Their efficient biosynthesis is often impeded by the limited substrate specificity and low in vivo activity of acyl‐CoA synthetases (ACSs) due to regulatory acylation of the catalytically important lysine residue in motif A10 (Lys‐A10). In this study, we identified an unusual ACS (UkaQ) from the UK‐2A biosynthetic pathway that naturally...
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