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A method to release plasmid DNA from Escherichia coli by heat treatment is presented. Heat treatment for 5 min at 95 o C and a combination of Triton X-100, EDTA to release plasmid DNA was performed under different conditions. The effects of key process parameters on the release of plasmid DNA and plasmid release kinetics were investigated. Cell disruption constant K x and plasmid degradation...
A medium for culturing Escherichia coli JM109 pcDNA3S as a DNA vaccine was developed using a stoichiometric model. In batch fermentation, using the designed medium MW1 and MW2, the plasmid DNA yield was 60+/-5.0 and 30+/-4.0 mg/l, respectively. However, plasmid the DNA yield was only 4+/-0.4 mg/l in LB medium and 20+/-3.5 mg/l in LBG medium. The plasmid stability characteristics of the JM109 pcDNA3S...
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