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In the present study, the expression of the μ-opioid receptor on protein level has been demonstrated in MCF7 breast cancer cells. Binding of the [ 125 I]-labeled μ-opioid receptor selective ligand endomorphin-2 (Tyr-Pro-Phe-Phe-NH 2 ) was examined in vitro using a cross-linking assay followed by a Western blot technique. The radioactive complex had a molecular weight of about 65kDa...
In the present study, we reported on the synthesis of two new μ-opioid peptide analogs, [d-1-Nal 3 ]morphiceptin and [d-1-Nal 4 ]-morphiceptin [1-Nal=3-(1-naphthyl)-alanine] which expressed receptor binding affinities at least at the level of the primary opioid ligands.The new analogs also labeled μ-opioid receptors on the cells of human breast cancer MCF-7 cell line with affinity...
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