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The multifunctional protein profilin is one of the most abundant proteins in the cytoplasm and is thought to regulate actin assembly and the phosphoinositide signaling pathway. Profilin binds to several different ligands including actin, poly-l-proline, and the head groups of polyphosphoinositides. Knowledge of profilin/ligand interactions is important for understanding the physiology of profilin...
By simultaneously incorporating in a protein 13 C-carbonyl- and 15 N-labeled amino acids with different levels of enrichment, characteristic asymmetric doublet-like patterns are observed for 15 N nuclei that are directly adjacent to the 13 C 1 -labeled residues, providing unambiguous identification of a large number of unique dipeptide fragments...
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