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We developed a novel technique for immobilizing enzymes on magnetic nanoparticle surfaces by using a methionine-tag system. Au/Fe-oxide composite nanoparticles synthesized through a radiochemical process were used as magnetic nanocarriers. The C-terminus of Tk-subtilisin, a model enzyme, was modified with a methionine tag and mixed with Au/Fe-oxide composite nanoparticles for immobilization via Au-S...
Immobilization of Au nanoparticles on super-paramagnetic iron-oxide (SPIO) enables facile and biocompatible surface functionalization via Au–S bond. Au/SPIO composite nanoparticle is easily modified by thiol-modified polyethylene glycol (PEG-SH), and they are successfully applied on MR tumor imaging. However, its large hydrodynamic size (~150 nm) still causes the accumulation to liver in vivo. In...
Au–Pd bimetallic nanoparticles supported on carbon particles were synthesized by reduction of precursor ions in an aqueous solution irradiated with a high energy electron beam. The composition of the samples was analyzed by the inductively coupled plasma atomic emission spectrometry (ICP-AES), and the morphology of the samples was observed by the transmission electron microscope (TEM). TEM micrographs...
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