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A new thiophenyl pyrazoline probe for Cu2+ in aqueous solution was synthesized and characterized by IR, NMR, HRMS and X-ray analysis. The probe displays remarkably high selectivity and sensitivity for Cu2+ with a detection limit of 1.919 × 10−7 M in aqueous solution (EtOH:HEPES = 1:1, v/v, 0.02 M, pH = 7.2). In addition, the probe is further successfully used to image Cu2+ in living cells and the...
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