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A new and simple method has been proposed to prepare magnetic Fe 3 O 4 -chitosan (CS) nanoparticles by cross-linking with sodium tripolyphosphate (TPP), precipitation with NaOH and oxidation with O 2 in hydrochloric acid aqueous phase containing CS and Fe(OH) 2 , and these magnetic CS nanoparticles were used to immobilize lipase. The effects on the sequence of adding...
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