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A novel yeast biocatalyst was prepared by using watermelon pieces as immobilizing support for yeast, Saccharomyces cerevisiae 101 strain for use in wine production. Immobilization was confirmed by electron microscopy. Suitability of biocatalyst was investigated by using it in repeated batch and continuous fermentations. The fermentation rate and other parameters were compared with free yeast cells...
The intracellular nitrilase of Nocardia globerula NHB-2 induced by propionitrile catalyses the hydrolysis of 3-cyanopyridine to nicotinic acid without detectable formation of nicotinamide. N. globerula NHB-2 showed highest conversion rate at 40mM 3-cyanopyridine, in 0.1M NaH 2 PO 4 /Na 2 HPO 4 buffer (pH 7.5) containing 5mM 2-mercaptoethanol. In 1l fed-batch reaction,...
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