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A label-free light-up fluorescent sensing strategy using hybridization chain reaction (HCR) amplification and DNA triplex assembly has been developed. Remarkably, the proposed fluorescence assay is successfully applied to the determination of avian influenza A (H7N9) virus DNA and thrombin. Herein, in the presence of targets, the target DNA/initiator triggers a cascade of hybridization events between...
A sensitive fluorescent strategy for sequence specific recognition of HIV dsDNA was established based upon Nicking Enzyme Signal Amplification (NESA) and triplex formation. dsDNA sequence from the site 7960 to site 7991 of the HIV1 dsDNA gene was designed as target dsDNA, which was composed of two complementary strands Oligonucleotide 1 with the sequence of 3′-CTT CCT TAT CTT CTT CTT CCA CCT CTC TCT...
A label-free method for sensitive detection of Hg 2+ was developed with gold nanoparticles (AuNPs) by using dynamic light scattering (DLS) technique. Oligonucleotide 5′-TTT CTT CTT CGT TGT TGT TT-3′ could transform from uncoil to rigid duplex or hairpin structure upon addition of Hg 2+ ions, which was confirmed by experiments of fluorescence resonance energy transfer and change of...
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