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Background Real-time RT-PCR has become a common and robust technique to detect and quantify low-abundance mRNA expression and is a prefered tool when examining fungal gene expression in infected host tissues. However, correct evaluation of gene expression data requires accurate and reliable normalization against a reference transcript. Thus, the identification of reference genes with stable expression...
Puccinia helianthi Schw. causes sunflower (Helianthus annuus L.) rust in many sunflower production regions in China. To study the virulence pattern of P. helianthi physiological races and evaluate the resistance of major sunflower hybrids and varieties to this pathogen, field surveys were undertaken in 33 counties across major sunflower production regions in China in 2013–2014. Eighty rust...
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