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Salmonella enterica is one of the most encountered causative pathogens of food-borne illnesses. Outbreaks of such diseases are commonly associated with the consumption of fresh fruits and vegetables. In this study, we report a simple colorimetric strategy for the detection of S. enterica Serovar Typhimurium in fresh-cut lettuce leaves based on polymerase chain reaction (PCR) products generated by...
A novel strategy was devised for colorimetric analysis of the products of the polymerase chain reaction (PCR). The method takes advantage of simultaneous amplification of a horseradish peroxidase-mimicking DNAzyme (HRPzyme) during the PCR process. It is performed using a DNA specific forward primer and a universal reverse primer containing a complementary HRPzyme sequence. The double-strand PCR products,...
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