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We studied pH regulation in freshly isolated rainbow trout hepatocytes using microspectrofluorometry with the fluorescent dye BCECF. In accordance with earlier data on rainbow trout hepatocytes, ion substitution (N-methyl D-glucamine for sodium and gluconate for chloride) and transport inhibitor [10M M methyl isobutyl amiloride (MIA) to inhibit sodium/proton exchange and 100M DIDS to inhibit bicarbonate...
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