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Human induced pluripotent stem cells (hiPSCs) are a true breakthrough in regenerative medicine with the potential to successfully treat many diseases, including orthopedic lesions, that are unresponsive to current treatments. However, chondrogenic differentiation in vitro is a poorly understood process and more research is needed. In this study, we compared the gene expression profile of chondrocyte-like...
The primary human dermal fibroblasts (PHDFs) from breast cancer patient were obtained to generate the human induced pluripotent stem cell line GPCCi001-A via lentiviral transfection. Thus, a modified EF1a-hSTEMCCA-loxP with tetO operator which regulates transgene expression was used. This method takes advantage of epigenetic regulation of transcription and allows for stable silencing of the reprogramming...
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